Coding

Part:BBa_K3831016:Design

Designed by: Tomáš Kotačka   Group: iGEM21_Brno_Czech_Republic   (2021-10-07)


sfGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 570
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sfGFP was fuzed with a localization tag and thus does not have start codon. It was codon-optimized for Bacillus subtilis and is in accordance with Assembly standards.


Source

FPbase

References